New detection of SARS-CoV-2 in two cats height months after COVID-19 outbreak appearance in France
| Autor: | Bertrand Boson, Nicolas Nesi, Alexandra Briend-Marchal, Eric M. Leroy, Matthieu Fritz, Solène Denolly, Serge G. Rosolen, Vincent Legros, Meriadeg Ar Gouil |
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| Rok vydání: | 2021 |
| Předmět: |
CATS
biology Coronavirus disease 2019 (COVID-19) medicine.diagnostic_test business.industry viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) fungi Outbreak Virology respiratory tract diseases Serology Microsphere body regions Immunoassay biology.protein medicine Antibody skin and connective tissue diseases business |
| DOI: | 10.1101/2021.03.24.436830 |
| Popis: | Although there are several reports in the literature of SARS-CoV-2 infection in cats, few SARS-CoV-2 sequences from infected cats have been published. In this report, SARS-CoV-2 infection was evaluated in two cats by clinical observation, molecular biology (qPCR and NGS), and serology (Microsphere immunoassay and seroneutralization). Following the observation of symptomatic SARS-CoV-2-infection in two cats, infection status was confirmed by RT-qPCR and, in one cat, serological analysis for antibodies against N-protein and S-protein, as well as neutralizing antibodies. Comparative analysis of five SARS-CoV-2 sequence-fragments obtained from one of the cats showed that this infection was not with one of the three recently emerged variants of SARS-CoV-2. This study provides additional information on the clinical, molecular, and serological aspects of SARS-CoV-2 infection in cats. |
| Databáze: | OpenAIRE |
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