The cytotoxicity of water-soluble carbon nanotubes on human embryonic kidney and liver cancer cells
| Autor: | Li Zhang, Yongzhen Yang, Xu-dong Su, Yuduan Gao, Lin Chen, Shiping Yu, Junli Wang, Jinglei Du, Xuguang Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Kidney
Materials science medicine.diagnostic_test Materials Science (miscellaneous) Cell technology industry and agriculture 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Molecular biology In vitro 0104 chemical sciences Flow cytometry chemistry.chemical_compound medicine.anatomical_structure chemistry Toxicity medicine General Materials Science Propidium iodide 0210 nano-technology Cytotoxicity Incubation |
| Zdroj: | New Carbon Materials. 33:36-45 |
| ISSN: | 1872-5805 |
| Popis: | The cytotoxicity of polyethylene glycol-modified carbon nanotubes (PEG-CNTs) on human embryonic kidney cells (293T) and hepatoma cells (HepG2) was studied in vitro. The effect of the concentration of PEG-CNTs on the cell activity and survival rate was detected by a colorimetric assay method after exposure for 24, 48 and 72 h. The dead HepG2 cells obtained by incubation in solutions with different concentrations of PEG-CNTs for 24 h can be stained by a dye, propidium iodide, and the cell mortality was determined by flow cytometry. Results show that the toxicity of PEG-CNTs with a good water solubility towards 293T and HepG2 cells increases with increasing PEG-CNT concentration. When the concentration of the PEG-CNTs is less than 100 μg/mL, the toxicity of the PEG-CNTs is Grade I (non-toxic) and when it is greater than 100 μg/mL but less than 200 μg/mL it is Grade II (mildly toxic), according to the ISO2109932-5 cytotoxicity standard. The toxicity grade does not change with increased time. |
| Databáze: | OpenAIRE |
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