Autor:	Emily Pisha, John M. Pezzuto
Rok vydání:	1997
Předmět:	chemistry.chemical_classification Phosphoric monoester hydrolases medicine.drug_class Phosphatase Cell Biology Biology Antiestrogen In vitro Enzyme chemistry Biochemistry Estrogen Cell culture medicine Alkaline phosphatase hormones hormone substitutes and hormone antagonists
Zdroj:	Methods in Cell Science. 19:37-43
ISSN:	1381-5741
DOI:	10.1023/a:1009746605060
Popis:	Methods are described for assessing the estrogenic or anti-estrogenic potential of test substances using cultured (human endometrial) Ishikawa cells. This cell line possesses an estrogen-inducible alkaline phosphatase, and estrogenic activity is indicated by assessing by the rate of p-nitrophenol generated from p-nitrophenylphosphate, subsequent to treatment with test compounds. Anti-estrogenic activity is indicated by a decrease in phosphatase activity when the cells are treated with test compounds in the presence of estrogen.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::50e402927124218131085782a2cdca98 https://doi.org/10.1023/a:1009746605060 Zobrazit plný text záznamu Full text from SpringerLink