ANTI-MOG ANTIBODIES

Autor: Ulrich Wurster, Ralf-Björn Lindert, Isabel Torens, Fedor Heidenreich
Rok vydání: 2007
Předmět:
DOI: 10.1016/b978-044452763-9/50076-7
Popis: There is a pressing need for biomarkers for diagnosis, prognosis and the potential efficiency of the diverse therapeutic options of the human autoimmune disease multiple sclerosis (MS). Antibodies to myelin oligodendrocyte glycoprotein (MOG) have been implicated to be involved in the pathogenesis of MS on the following considerations: (i) localization of MOG on the external surface of myelin, (ii) presence of abs to MOG peptides and protein in demyelinating plaques, and (iii) striking similarity in the disease course and pathological picture between MS and the MOG-induced animal disease experimental allergic encephalitis. Most research on MOG abs has been carried out with denatured antigen preparations representing the extracellular part of MOG by ELISA and Western Blot (WB). Such assays will detect primarily abs to linear epitopes, which are rarely pathogenic and may have arisen as a result of myelin breakdown. Depending on the assay conditions, the isotype IgG or IgM, and the definition of the cut-off frequencies from 38 to 100% have been reported. In contrast, positive results with fluid phase methods like FACS or RIA and native antigen stayed below 10%. The high rates of WB MOG positives among healthy controls prevent its use for diagnostic purposes, but a recent report suggested excellent performance as a prognostic test. However, up to now four follow-up studies have been unable to confirm the original optimistic results. The inherent problems of the WB MOG assay raise serious doubts if it will ever be applicable for routine patient testing.
Databáze: OpenAIRE
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