Sensitive fluorescence detection in capillary electrophoresis using laser diodes and multiplex dyes
| Autor: | Malte Köllner, K.-T. Han, K. H. Drexhage, Stefan Seeger, R. Müller, Jutta Arden-Jacob, Jürgen Wolfrum, Markus Sauer, G. Bachteler |
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| Rok vydání: | 1994 |
| Předmět: |
Detection limit
Laser diode Biophysics Analytical chemistry General Chemistry Condensed Matter Physics Laser Biochemistry Fluorescence Atomic and Molecular Physics and Optics Fluorescence spectroscopy law.invention Rhodamine chemistry.chemical_compound Capillary electrophoresis chemistry law Laser-induced fluorescence |
| Zdroj: | Journal of Luminescence. 62:101-108 |
| ISSN: | 0022-2313 |
| DOI: | 10.1016/0022-2313(94)90336-0 |
| Popis: | A detector for laser induced fluorescence based on laser diole technology was designed and sensitivity measurements with rhodamine 800 were performed. The detection limit is around 100 molecules in the detection volume. Using the laser diode in combination with a commercial capillary electrophoresis system, about 1200 molecules were detectable. Using time-resolved fluorescence measurements multiplex dyes were recognized with a pulsed laser diode and a pattern recognition technique collecting only a few hundred photons. |
| Databáze: | OpenAIRE |
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