| Autor: |
Jianfeng Sun, Martin Philpott, Danson Loi, Shuang Li, Pablo Monteagudo-Mesas, Gabriela Hoffman, Jonathan Robson, Neelam Mehta, Vicki Gamble, Tom Brown, Tom Brown Sr, Stefan Canzar, Udo Oppermann, Adam P Cribbs |
| Rok vydání: |
2023 |
| Popis: |
Unique Molecular Identifiers (UMIs) are random oligonucleotide sequences that remove PCR amplification biases. However, the impact that PCR associated sequencing errors have on the accuracy of generating absolute counts of RNA molecules is underappreciated. We show that PCR errors are the main source of inaccuracy in both bulk and single-cell sequencing data, and synthesizing UMIs using homotrimeric nucleotide blocks provides an error correcting solution, that allows absolute counting of sequenced molecules. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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