Differential gene expression of the B Cell Receptor (BCR) signaling pathway during pregnancy in mice
| Autor: | Natalin Jimena Valeff, Damian Muzzio, Martin Abba, Marek Zygmunt, Federico Jensen |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 202:123.19-123.19 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.202.supp.123.19 |
| Popis: | The aim of this work was to characterize BCR signaling pathway gene expression in B cells during pregnancy. B-cell receptor (BCR) signaling regulates critical cell-fate decisions in B cells like activation, proliferation, expression of costimulatory molecules and cytokine receptors. Changes on the dynamic of BCR signaling pathway during pregnancy might have an impact on the way how the immune system fight against infections or regulate autoimmunity during this critical period of time. We performed a genome-wide transcriptome profile (GeneChip™ Mouse Gene 1.0 ST Array, Affymetrix) on pure splenic B cells isolated from pregnant and non-pregnant mice. Results were analyzed and significant gene expression regulation was confirmed by qPCR. In silico analysis showed that genes associated with B cell receptor signaling pathway were significantly down-regulated in B cells from pregnant mice compared to non-pregnant animals (≈1.5–2 fold changes, Limma eBayes test, p We showed here that genes associated with BCR signaling leading to B cell activation and proliferation were significantly down-regulated during pregnancy. These results might help to understand why pregnant women are more susceptible to infections as well as some B cell associated autoimmune diseases experience a significant improvement in the symptoms during pregnancy. |
| Databáze: | OpenAIRE |
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