Delivery of Barnase to Cells in Liposomes Functionalized by Her2-Specific DARPin Module
| Autor: | E. I. Shramova, M. V. Shilova, G. M. Proshkina, Victoria O. Shipunova, Alexey A. Schulga, S. M. Deyev |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Barnase Liposome biology 010405 organic chemistry Chemistry Organic Chemistry 01 natural sciences Biochemistry 0104 chemical sciences Cell membrane 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure DARPin biology.protein medicine Biophysics Cytotoxic T cell Ribonuclease skin and connective tissue diseases Bacterial outer membrane Cytotoxicity neoplasms |
| Zdroj: | Russian Journal of Bioorganic Chemistry. 46:1156-1161 |
| ISSN: | 1608-330X 1068-1620 |
| DOI: | 10.1134/s1068162020060308 |
| Popis: | In this work for the first time we demonstrate the possibility of ribonuclease barnase delivery to HER2-positive human cancer cells in a liposomal form. The principle of loading liposomes with ribonuclease is based on the electrostatic interaction of the protein with the inner surface of the liposomes. The specificity of liposomes to the HER2-receptor is determined by the presence of the anti-HER2-address module DARPin on the outer membrane of the liposome. It was shown that cytotoxicity specific for HER2-positive cells is determined precisely by the presence of an address HER2-specific module on the surface of liposomes, and the severity of the cytotoxic effect correlates with the level of expression of the HER2 receptor on the cell membrane. |
| Databáze: | OpenAIRE |
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