Electroretinogram analysis of zebrafish retinal function across development
| Autor: | Jennifer C. Hocking, Nathan J. Nadolski, Casey X. L. Wong |
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| Rok vydání: | 2020 |
| Předmět: |
Retina
animal structures genetic structures fungi Biology biology.organism_classification eye diseases Sensory Systems 03 medical and health sciences Ophthalmology 0302 clinical medicine medicine.anatomical_structure Visual function Physiology (medical) 030221 ophthalmology & optometry medicine Retinal function sense organs Scotopic vision Ocular disease Neuroscience Zebrafish Erg 030217 neurology & neurosurgery Photopic vision |
| Zdroj: | Documenta Ophthalmologica. 142:99-109 |
| ISSN: | 1573-2622 0012-4486 |
| DOI: | 10.1007/s10633-020-09783-y |
| Popis: | The electroretinogram (ERG) is a powerful approach for investigating visual function in zebrafish ocular disease models. However, complexity, cost, and a literature gap present as significant barriers for the introduction of this technology to new zebrafish laboratories. Here, we introduce a simplified and effective method to obtain zebrafish ERGs. In-house assembled recording electrodes and a custom 3D-printed platform were used to gather high-quality and consistent ERG data from zebrafish at 3 developmental timepoints—larval, juvenile, and adult. Fish were tested under both scotopic (dark-adapted) and photopic (light-adapted) conditions to differentiate between the rod and cone systems, respectively. Robust ERG waveforms across all developmental timepoints were obtained using the methodology presented here. We observed an overall increase in signal amplitude as development progressed, reflecting maturation of the zebrafish retina. Oscillatory potentials could also be isolated from the generated waveforms. This simplified approach to the zebrafish ERG can generate waveforms comparable to the existing approaches and helps reduce barriers for zebrafish laboratories studying ocular development and disease. |
| Databáze: | OpenAIRE |
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