| Autor: |
L. J. Brown, D.G. Steffen, D.R. Rao, H.J. Mersmann, J.M. Houk |
| Rok vydání: |
1977 |
| Předmět: |
|
| Zdroj: |
International Journal of Biochemistry. 8:773-779 |
| ISSN: |
0020-711X |
| DOI: |
10.1016/0020-711x(77)90031-3 |
| Popis: |
1. 1. The ATP:citrate lyase from swine adipose tissue can be assayed using conditions to produce either linear or biphasic double-reciprocal plots of citrate saturation curves; the former had lesser velocity than the latter. 2. 2. The ionic forms of the substrate were probably Mg·ATP 2− and either Mg·citrate − or citrate 3− . There was little effect of ATP 4− and inhibition by high Mg 2+ concentrations. 3. 3. The enzyme was inhibited by hydroxycitrate and by ADP, both probably as the magnesium complexes. 4. 4. The ionic form of the inhibitor, tricarballylate, was uncertain. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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