| Autor: |
A Wouters, Ilse Kersters, Joris Mergaert, Laurent Verschuere, Luc Vauterin, Lieven Van Vooren, Willy Verstraete, Jean Swings |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Systematic and Applied Microbiology. 20:439-447 |
| ISSN: |
0723-2020 |
| DOI: |
10.1016/s0723-2020(97)80013-6 |
| Popis: |
Summary The Biolog system was evaluated as a simple and rapid assay to characterize heterotrophic microbial communities. Factors influencing the substrate utilization profiles of microbial communities present in surface water and compost material were determined. Examination of five different production batches of Biolog GN microplates showed that the color development in the substrate response wells is significantly affected by the production batch. Although community Biolog profiles within the same production batch of GN microplates were reproducible, the color development in replicate control wells contained in Biolog MT microplates varied significantly. This implies that several substrate-free reference wells need to be inoculated with the same environmental sample to allow reliable data transformations of the 95 raw color responses in the GN microplates. Replicate suspensions of compost material could not be distinguished from each other using Biolog GN microplates originating from the same production batch, suggesting that the sample preparation procedure was highly reproducible. However, the data indicate that reliable use of the Biolog assay for the characterization of heterotrophic microbial communities is only possible by comparing samples of equivalent inoculum densities. Also, multiple readings over a time course of incubation are needed, to overcome the effect of nonlinear color development. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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