Morphological and molecular identification of airborneFusariumpropagules trapped in a maize field in Hungary

Autor:	Zsuzsanna Koncz, Donát Magyar, Árpád Szécsi
Rok vydání:	2011
Předmět:	Fusarium Air sampling Pcr assay food and beverages Plant Science Biology biology.organism_classification chemistry.chemical_compound chemistry Propagule Insect Science Specific primers Fumonisin Botany Pcr analysis Molecular identification
Zdroj:	Acta Phytopathologica et Entomologica Hungarica. 46:175-184
ISSN:	1588-2691 0238-1249
DOI:	10.1556/aphyt.46.2011.2.1
Popis:	Airborne propagules of Fusarium spp. were collected on Fusarium selective medium with Andersen sampler in maize field. Fusarium isolates were identified based on morphological characters and using speciesspecific primersVERT 1/2, PRO 1/2 and SUB 1/2. The PCR analysis confirmed morphological identification of F. verticillioides, F. proliferatum and F. subglutinans. The VERTF 1/2 set of primers were used to discriminate potential fumonisin-producing strains of F. verticillioides, and all F. verticillioides strains scored positive for VERTF 1/2 pair of primers. This is the first study where specific primers were used for the confirmation of morhological determination of the above-mentioned Fusarium species, and selection of fumonisin-producing airborne isolates of F. verticillioides in Hungary.
Databáze:	OpenAIRE
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