Concentration and Distribution of Neuropeptide Y, Galanin, β-endorphin, Vasoactive Intestinal Peptide and Gonadotrophin-Releasing Hormone in the Hypothalamus of Gilts during Oestrogen-Induced Surge Secretion of Luteinizing Hormone

Autor: Adam J. Ziecik, M. Lakomy, H. Kalamarz, R R Kraeling, S. Okrasa, W. Sienkiewicz
Rok vydání: 1999
Předmět:
Zdroj: Reproduction in Domestic Animals. 34:477-487
ISSN: 1439-0531
0936-6768
Popis: Contents The relationship of neuropeptide Y (NPY), galanin (GAL), β-endorphin (β-END) and vasoactive intestinal peptide (VIP) to GnRH neurons were determined during the estradiol-induced LH surge. In experiment 1, 16 ovariectomized (OVX) gilts received 15 μg estradiol benzoate (EB)/kg BW at 0800 h and were slaughtered at either 24 h (n = 5), 48 h (n = 6) or 72 h (n = 5) later and five were injected with corn oil vehicle (0 h controls). Concentrations of neuropeptides were determined in tissue extracts by RIA. In experiment 2, nine OVX gilts were injected with EB as in experiment 1 and killed at either 24, 48 or 72 h (n = 3) later and three were not injected with EB (0 h controls). Frozen sections were processed to localize neuropeptides. In experiment 1, all measured neuropeptides were highest in pituitary stalk median eminence (SME). The GnRH concentration was not different at any time point in medial basal hypothalamus (MBH), preoptic area (POA) or SME. The NPY content in MBH was lower at 24, 48 and 72 h after EB than at 0 h (p
Databáze: OpenAIRE
Externí odkaz: