| Popis: |
Scientists continue to investigate photosynthesis—nature’s process to efficiently regulate and store energy. To explore the mechanisms of regulation we used fluorescence, resonance Raman spectroscopy, and biochemical preparative methods. For detailed analysis we applied decomposition of the low temperature steady‐state fluorescence spectra. This allowed us to estimate the emission of distinct pigment protein complexes (PPC) and evaluate the fluorescence of various aggregation forms of the main light harvesting complex (LHCII), which plays a major role in the studied mechanisms. Resonance Raman spectroscopy revealed with precision the relation between changes in pigments’ spectral properties and structural distortions of PPC. It was shown that aggregation of LHCII led to out‐of‐plane distortion, not only of neoxanthin, but also of lutein molecules. This was enhanced when the complex was embedded in thylakoid membranes (TM). It was suggested that lutein molecules are more closely related to the process of a... |
