Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues
| Autor: | Wai W. Mon, Tin Mar Lynn, San S. Yu, Ei Phyu Kyaw, May T. Nwe, Zaw Ko Latt, Kyaw Nyein Aye |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Arabinose General Immunology and Microbiology biology Cellulase biology.organism_classification 01 natural sciences Reducing sugar 03 medical and health sciences chemistry.chemical_compound Azomonas agilis 030104 developmental biology chemistry biology.protein Nitrogen fixation Ammonium Food science Cellulose Bacteria 010606 plant biology & botany |
| Zdroj: | The Open Microbiology Journal. 12:154-162 |
| ISSN: | 1874-2858 |
| DOI: | 10.2174/1874285801812010154 |
| Popis: | Introduction:Azomonas agilis, a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar.Methods & Materials:The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulolytic activity was screened by plat assay and detected by Dinitrosalicyclic acid method (DNS).Results & Discussion:The isolatedA. agilisgrew in media containing 3-12% of NaCl, although the growth became poor when NaCl concentrations increased. Among various carbon sources, sucrose was the best source for ammonium accumulation of this bacterium, whereas arabinose was not the suitable carbon source. Although the nitrogen-fixing activity ofA. agiliswas highest after one week incubation, cellulase enzyme production was highest after 2-3 days of incubation. It was observed that cellulase enzyme activity ofA. agilisfor cellulose and sodium carboxymethyl cellulose (CMC) was almost the same. Three agricultural wastes were used to detect the cellulase enzyme activity ofA. agilis, cellulase activity was better on filter paper as a substrate when compared to rice-straw and sawdust.Conclusion:So, the isolatedA. agilishas high potential as an effective bacterial strain to use in sustainable agriculture and degradation of some agricultural residues. |
| Databáze: | OpenAIRE |
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