The Chicken Egg Chorioallantoic Membrane (CAM) Model as an In Vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells
| Autor: | Heike Allgayer, Jörg H. Leupold, Nitin Patil |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
animal structures Intravasation Alu element Embryo Biology medicine.disease Metastasis Cell biology 03 medical and health sciences Chorioallantoic membrane 030104 developmental biology 0302 clinical medicine Real-time polymerase chain reaction In vivo medicine Primer (molecular biology) 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071613498 |
| Popis: | The CAM model enables an in vivo analysis of the individual sub-steps of the metastatic cascade like local invasion, intravasation, or the establishment of metastasis in particular organs. Incubated fertilized chicken eggs are inoculated with human tumor cells and further processed for up to 9-10 days. The invasion and metastasis of these cells is then detected quantitatively with high specificity and sensitivity by means of a PCR for human ALU sequences, using the genomic DNA isolated from distant portions of the CAM, as well as from diverse internal organs of the developing embryo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|