The Relationship between Steroidogenic Factor 1 and DAX-1 Expression and in Vitro Gonadotropin Secretion in Human Pituitary Adenomas1
| Autor: | Ashley B. Grossman, D. F. Wood, Simon Aylwin, Jacky M. Burrin, John P Monson, G. M. Besser, J. P. Welch, C. L. Davey, J. F. Geddes |
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| Rok vydání: | 2001 |
| Předmět: |
Steroidogenic factor 1
endocrine system medicine.medical_specialty Messenger RNA Pituitary gland Adenoma Somatotropic cell medicine.drug_class Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry Biology medicine.disease Biochemistry Gonadotropin secretion Endocrinology medicine.anatomical_structure Internal medicine Gene expression Cancer research medicine Gonadotropin hormones hormone substitutes and hormone antagonists |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 86:2476-2483 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.86.6.7531 |
| Popis: | The orphan nuclear receptors, steroidogenic factor 1 (SF-1) and DAX-1, are involved in gonadotroph differentiation, and SF-1 has been shown to activate the LH-β and glycoprotein hormone α-subunit (αGSU) gene promoters. Pituitary adenomas from 34 patients [13 somatotroph tumors, 4 prolactinomas, and 17 clinically nonfunctioning pituitary adenomas (NFPAs)] were enzymatically dispersed and cultured in vitro for 48 h. Tissue culture medium was collected and assayed for LH, FSH, and αGSU; messenger RNA was extracted from adherent cells, and expression of SF-1 and DAX-1 messenger RNA was determined by RT-PCR and verified by direct DNA sequencing. The presence of DAX-1 protein in tumor tissue was confirmed by immunocytochemistry.DAX-1 was demonstrated in all NFPAs, 7 of 13 somatotroph tumors and 0 of 4 prolactinomas. SF-1 expression occurred in 8 of 16 NFPAs, 4 of 12 somatotroph tumors, and 1 of 4 prolactinomas. LH secretion in vitro was greater in NFPAs that were SF-1 positive (P < 0.05). Neither FSH secretion nor αGSU secretion in vitro were significantly related to the expression of SF-1 or DAX-1. SF-1-positive somatotroph tumors immunostained positively for LH-β and/or FSH-β and secreted gonadotropins in vitro.SF-1 expression is associated with the in vitro secretion of LH by NFPAs. A proportion of somatotroph tumors also express SF-1 and DAX-1 and secrete gonadotropin hormones in vitro. |
| Databáze: | OpenAIRE |
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