Image scanning fluorescence emission difference microscopy based on a detector array
Autor: | Shaocong Liu, Diyi Liu, Zhanghao Ding, Xiaoli Liu, Yongfu Li, Cuifang Kuang, Shiyi Sun |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Histology Photon Materials science business.industry Confocal Resolution (electron density) Analytical chemistry 01 natural sciences Fluorescence Pathology and Forensic Medicine law.invention 010309 optics 03 medical and health sciences Transverse plane 030104 developmental biology Optics Confocal microscopy law 0103 physical sciences Microscopy Fluorescence microscope business |
Zdroj: | Journal of Microscopy. 266:288-297 |
ISSN: | 0022-2720 |
Popis: | We propose a novel imaging method that enables the enhancement of three-dimensional resolution of confocal microscopy significantly and achieve experimentally a new fluorescence emission difference method for the first time, based on the parallel detection with a detector array. Following the principles of photon reassignment in image scanning microscopy, images captured by the detector array were arranged. And by selecting appropriate reassign patterns, the imaging result with enhanced resolution can be achieved with the method of fluorescence emission difference. Two specific methods are proposed in this paper, showing that the difference between an image scanning microscopy image and a confocal image will achieve an improvement of transverse resolution by approximately 43% compared with that in confocal microscopy, and the axial resolution can also be enhanced by at least 22% experimentally and 35% theoretically. Moreover, the methods presented in this paper can improve the lateral resolution by around 10% than fluorescence emission difference and 15% than Airyscan. The mechanism of our methods is verified by numerical simulations and experimental results, and it has significant potential in biomedical applications. |
Databáze: | OpenAIRE |
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