A proteolytic enzyme associated with the infection of cocoa beans byBotryodiplodia theobromae

Autor:	B. Fessehatzion, P.O. Olutiola
Rok vydání:	1987
Předmět:	chemistry.chemical_classification Ammonium sulfate Protease Iodoacetic acid medicine.diagnostic_test Proteolysis medicine.medical_treatment Proteolytic enzymes food and beverages Biology Microbiology chemistry.chemical_compound Enzyme chemistry Biochemistry Casein Genetics medicine Ammonium Molecular Biology
Zdroj:	FEMS Microbiology Letters. 43:67-70
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1987.tb02099.x
Popis:	Cocoa beans infected by Botryodiplodia theobromae produced proteins which possessed proteolytic activity. The enzyme was partially purified by ammonium sulphate precipitation followed by molecular exclusion and ion-exchange chromatography. The Mr was approx. 80000. The enzyme exhibited maximum activity at pH 8.0 and 35°C, and possessed an apparent Km of approx. 8.3 mg/ml for casein. Activity of the enzyme was stimulated by Ca2+ and Mg2+ but inhibited by mercuric chloride, EDTA, iodoacetic acid and 2,4-dinitrophenol.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5084c8def3a66277f6b8dda7eaa49098 https://doi.org/10.1111/j.1574-6968.1987.tb02099.x Zobrazit plný text záznamu Plný text