| Popis: |
The objective of this study was to develop a rapid and sensitive UV spectroscopic and RP-HPLC method for the analysis of Amlodipine Besylate and Indapamide in bulk drug and pharmaceutical dosage form by using the most commonly employed C-18 column with UV-detection. Acetonitrile: acetate buffer pH-5 (40: 60 % v/v) with 1.2 mL / min flow rate was selected as mobile phase.In the HPLC method, the conditions were optimized to obtain an adequate separation of components. Initially, various mobile phase compositions were tried to separate active ingredients. This method has been found to be better than previously reported methods, due to its wider range of linearity, use of readily available mobile phase, lack of extraction procedures. Hence thesemethodscould be used in quality control for routine analysis of finished products of amlodipine besylate and Indapamide simultaneously without any interference. |
