Modified merozoite surface protein-1 peptides with short alpha helical regions are associated with inducing protection against malaria
| Autor: | Mary Helena Torres, Manuel E. Patarroyo, Raul Rodriguez, Yolanda Silva, Magnolia Vanegas, Fanny Guzmán, Luz Mary Salazar, Jaiver E. Rosas |
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| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | European Journal of Biochemistry. 270:3946-3952 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1046/j.1432-1033.2003.03780.x |
| Popis: | The merozoite surface protein-1 represents a prime candidate for development of a malaria vaccine. Merozoite surface protein-1 has been shown to demonstrate high-activity peptide binding to human red blood cells. One of the high-activity binding peptides, named 5501, located in the N-terminus (amino acid sequence MLNISQHQCVKKQCPQNS) of the 19-kDa molecular mass fragment of merozoite surface protein-1, is conserved, nonimmunogenic and nonprotective. Its critical binding residues were identified and replaced with amino acids of similar mass but different charge, in order to modify their immunogenic and protective characteristics. Three analogues with positive or negative immunological results were studied by nuclear magnetic resonance to correlate their three-dimensional structure with their biological functions. The studied peptides presented α-helical fragments, but in different peptide regions and extensions, except for randomly structured 5501. We show that altering a few amino acids induced immunogenicity and protectivity against experimental malaria and changed the peptide three-dimensional structure, suggesting a better fit with immune-system molecules. |
| Databáze: | OpenAIRE |
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