Gene delivery to the heart by magnetic nanobeads
| Autor: | Nan Ma, Zhaohui Zhou, Guping Tang, Andreas Lendlein, Catharina Nesselmann, John Wang, Christof Stamm, Andreas Liebold, Alexander Kaminski, Ferenc Öri, Karola Lützow, Lee-Lee Ong, Wenzhong Li, Gustav Steinhoff |
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| Rok vydání: | 2007 |
| Předmět: |
technology
industry and agriculture Gene targeting macromolecular substances Transfection Conjugated system Gene delivery equipment and supplies Condensed Matter Physics Electronic Optical and Magnetic Materials chemistry.chemical_compound Nuclear magnetic resonance chemistry In vivo Biophysics Magnetic nanoparticles human activities Linker DNA |
| Zdroj: | Journal of Magnetism and Magnetic Materials. 311:336-341 |
| ISSN: | 0304-8853 |
| DOI: | 10.1016/j.jmmm.2006.11.201 |
| Popis: | Gene delivery with non-viral gene vectors to the cardiovascular system suffers from low transfection efficiency. In this study, magnetic fields were investigated to assist cardiovascular gene delivery via magnetic nanobeads both in vitro and in vivo. The magnetic field was provided with a 1120 mT Nd–Fe–B permanent magnet while complexes of poly-ethyleneimine (PEI) and various DNA plasmids were conjugated with magnetic nanobeads (MNB) using a Sulfo-NHS-LC-Biotin linker. In vitro results showed that transfection in two cell lines was 30–80-fold higher in magnetically conjugated MNB/PEI/DNA complexes than transfection from PEI/DNA complexes alone. Similarly, in vivo results using mouse models showed 72 h after injection observable gene expression in the heart with conjugated MNB/PEI/DNA complexes, but barely with PEI/DNA complexes alone. |
| Databáze: | OpenAIRE |
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