Comparison of pancreas specific promoters from mouse and rat genome
| Autor: | Balázs Németh, Péter Hegyi, Miklos Sahin-Toth |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Messenger RNA Hepatology business.industry Endocrinology Diabetes and Metabolism Stomach Gastroenterology medicine.disease digestive system Molecular biology Endocrinology medicine.anatomical_structure Downregulation and upregulation Internal medicine Gene expression Collagenase Medicine Pancreatitis business Digestion Pancreas hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Pancreatology. 15:S51 |
| ISSN: | 1424-3903 |
| DOI: | 10.1016/j.pan.2015.05.203 |
| Popis: | s / Pancreatology 15 (2015) S1eS141 S51 stomach and identified in the pancreas. GHRL has been demonstrated to protect pancreatic tissue from caerulein-induced pancreatitis (CIP). Aims: To investigate the effect of GHRL and caerulein on gene expression and on protein signals of GHS-R1a, GHRL and of an antioxidant enzyme; superoxide dysmutase (SOD) -3 in the isolated pancreatic acini. Materials & methods: Pancreatic acini were isolated by collagenase digestion from control rats and from rats pretreated with GHRL (50 ug/kg i. p.) administered 48 hours before the isolation, then the acinar cells were stimulated by using of caerulein (10-8M). The gene expression were determined by RT-PCR, whereas the protein contents were assessed employing Western-blot. Results: Protein expressions and mRNA signals for GHS-R1a, GHRL and for SOD-3 have been detected in the pancreatic acini under basal conditions and have been significantly upregulated following application of GHRL to the rats. Caerulein markedly downregulated signals GHS-R1a and SOD-3 in pancreatic acini, but failed to affect these for GHRL. Pretreatment of rats with GHRL reversed caerulein-induced suppression of GHS-R1a and SOD-3 signals. Conclusion: Caerulein is able to modify the GHS-R1a in the pancreatic acini and this effect could be prevented by pretreatment of rats with GHRL. Beneficial effect of GHRL could be dependent, at least in part, on the activation of SOD-3 and improvement of antioxidant system in the pancreas. |
| Databáze: | OpenAIRE |
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