Cation Activation of the Basolateral Sodium-Potassium Pump in Turtle Colon

Autor:	D C Dawson, Dan R. Halm
Rok vydání:	1983
Předmět:	Physiology Sodium Potassium Kinetics chemistry.chemical_element Apical membrane Rubidium Red blood cell medicine.anatomical_structure chemistry Biochemistry medicine Biophysics Na+/K+-ATPase Epithelial polarity
Zdroj:	Journal of General Physiology. 82:315-329
ISSN:	1540-7748 0022-1295
DOI:	10.1085/jgp.82.3.315
Popis:	The current generated by electrogenic sodium-potassium exchange at the basolateral membrane of the turtle colon can be measured directly in tissues that have been treated with serosal barium (to block the basolateral potassium conductance) and mucosal amphotericin B (to reduce the cation selectivity of the apical membrane). We studied the activation of this pump current by mucosal sodium and serosal potassium, rubidium, cesium, and ammonium. The kinetics of sodium activation were consistent with binding to three independent sites on the cytoplasmic side of the pump. The pump was not activated by cellular lithium ions. The kinetics of serosal cation activation were consistent with binding to two independent sites with the selectivity Rb > K > Cs > NH4. The properties and kinetics of the basolateral Na/K pump in the turtle colon are at least qualitatively similar to those ofthe well-characterized Na/K-ATPase of the human red blood cell .
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::501db6776c7547981e485252815cbb6b https://doi.org/10.1085/jgp.82.3.315 Zobrazit plný text záznamu