Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors
| Autor: | Yukihisa Shimada, Akiko Sato, Kazuo Soeno |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification biology Chemistry fungi Mutant food and beverages Endogeny biology.organism_classification 01 natural sciences Reverse genetics In vitro 03 medical and health sciences 030104 developmental biology Biochemistry Auxin In vivo Arabidopsis heterocyclic compounds Chemical genetics 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071609538 |
| Popis: | Auxin plays important roles in almost all aspects of plant growth and development. Chemical genetics is an effective approach to understand auxin action, especially in nonmodel plant species, in which auxin-related mutants are not yet available. Among auxin-related chemical tools, we present approaches to utilize auxin biosynthesis inhibitors. The inhibitors are effective not only to understand auxin biosynthesis but also to understand auxin action. The effectiveness of the inhibitors can be assessed based on in vitro or in vivo assays. The in vitro assay employs enzyme inhibition assays. The in vivo assay employs UPLC-MS/MS-based analysis of endogenous IAA and its intermediates or metabolites. |
| Databáze: | OpenAIRE |
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