Antilipolytic Actions of Vanadate and Insulin in Rat Adipocytes Mediated by Distinctly Different Mechanisms1

Autor:	Gerard Elberg, Natesampillai Sekar, Jinping Li, Zhi Bin He, Yoram Shechter
Rok vydání:	1997
Předmět:	medicine.medical_specialty Insulin medicine.medical_treatment Phosphatase Protein tyrosine phosphatase Okadaic acid Wortmannin chemistry.chemical_compound Endocrinology chemistry Internal medicine medicine Lipolysis Vanadate
Zdroj:	Endocrinology. 138:2274-2279
ISSN:	1945-7170 0013-7227
Popis:	Vanadate, which mimics the biological effects of insulin, also inhibits lipolysis in rat adipocytes. Here we demonstrate that the antilipolytic effect of vanadate differs from that of insulin at least by the five following criteria: 1) vanadate inhibits lipolysis mediated by high (supraphysiological) concentrations of catecholamines; 2) vanadate antagonizes (Bu)2cAMP-mediated lipolysis; 3) vanadate antagonizes isobutylmethylxanthine-dependent lipolysis, 4) vanadate inhibits lipolysis mediated by okadaic acid; and 5) wortmannin, which blocks the antilipolytic effect of insulin, fails to block vanadate-mediated antilipolysis. Vanadate does activate phosphoinositol 3-kinase, and wortmannin blocks this activation. Our working hypothesis assumes that all of the insulin-like effects of vanadate, including antilipolysis, are initiated by the inhibition of protein phosphotyrosine phosphatases (PTPases). Among documented PTPase inhibitors we found that VOSO4 (oxidation state +4), several organic vanadyl compounds ...
Databáze:	OpenAIRE
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