Obtaining and Culturing Haploid Cells

Autor: Rudolf Endress
Rok vydání: 1994
Předmět:
Zdroj: Plant Cell Biotechnology ISBN: 9783642081651
DOI: 10.1007/978-3-662-02996-1_4
Popis: Haploid cells may be obtained either by in vitro androgenesis, i.e. the culture of anthers, pollen or microspores (Brassica) (Guha and Maheshwari 1964), or by in vitro gynogenesis, the culture of unfertilized egg cells, cells of the embryo sac (synergids, antipods, central cells) or the ovary (Yang and Zhou 1990). However, usually only a small percentage of the explants reacts by initiating direct embryogenesis. If callus is initially formed, diploid, polyploid and aneuploid cells develop as well. The sporophytes regenerated from these cells have a gametophytic chromosome constitution.
Databáze: OpenAIRE