| Autor: |
J.P. Truchot, A. Rtal, L. Nonnotte |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Aquatic Toxicology. 36:239-252 |
| ISSN: |
0166-445X |
| DOI: |
10.1016/s0166-445x(96)00805-3 |
| Popis: |
Copper of exogenous origin is present in crab hemolymph mainly in a protein-bound form. Various approaches were used to assess the toxicity of this protein-bound exogenous metal. Mortality due to copper directly injected into the hemolymph space was apparent at circulating concentrations of non-hemocyanic copper much higher than that (about 150 μM) attained during exposure to a lethal level of waterborne copper (31.5 μM in water). No cytological damage to the gill epithelium was apparent in crabs injected with the metal to attain a hemolymph exogenous copper level of 150 μM, in contrast to those observed at similar hemolymph concentrations during waterborne metal exposure. The cytotoxicity of copper added to an in vitro hepatopancreatic cell preparation was greatly diminished in the presence of hemolymph proteins, probably in relation to a reduced copper uptake by these cells. Thus, binding of copper to hemolymph proteins and particularly hemocyanin plays a significant role, not only for transport and distribution, but also for detoxification of the metal within the organism. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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