| Popis: |
Background: Feline parvovirus (FPV) is a member of the Parvoviridae family, which is a major enteric pathogen of cats worldwide. This study aimed to investigate the prevalence of feline parvovirus in Beijing, China and analyze the genetic features of detected viruses.Results: In this study, a total of 60 parvovirus-positive samples (8.5%) were detected from 702 cat fecal samples using parvovirus specific PCR. The complete VP2 genes were amplified from all these samples. Among them, 55 sequences were characterized as FPV (91.7%), the other five were typed as canine parvovirus type 2 (CPV-2) variants (8.3%), comprised of four CPV-2c and a new CPV-2b strain. In order to identify the origin of CPV-2 variants in cats, we amplified full-length VP2 genes from 7 fecal samples of dogs infected with CPV-2, which were further classified as CPV-2c. Moreover, the sequences of new CPV-2b/MT270586 and CPV-2c/MT270587 detected from feline samples shared 100% identity with previous canine isolates KT156833 and MF467242 respectively, indicating the CPV-2 variants circulating in cats were derived from dogs. Sequence analysis indicated new mutations, Ala91Ser and Ser192Phe, in the FPV sequences, while obtained CPV-2c carried mutations reported in Asian CPV variants, showing they share a common evolutionary pattern with the Asian 2c strains. Interestingly, the FPV sequence (MT270571), displaying four CPV-specific residues, was found to be a putative recombinant sequence between CPV-2c and FPV. Phylogenetic analysis of the VP2 gene showed that amino acid and nucleotide mutations promoted the evolution of different geographical branches of FPV and CPV lineages.Conclusions: Our findings will be helpful to further understand the circulation and evolution of canine and feline parvovirus in Beijing. |
