Expression of the Human p55 and p75 Tumor Necrosis Factor Receptors in Primary Villous Trophoblasts and Their Role in Cytotoxic Signal Transduction1
| Autor: | Denise G. Hemmings, Larry J. Guilbert, Jane Yui, M. Garcia-Lloret |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Programmed cell death medicine.medical_treatment Trophoblast Cell Biology General Medicine Biology Cell biology Cytokine medicine.anatomical_structure Endocrinology Reproductive Medicine Apoptosis Internal medicine embryonic structures medicine Tumor necrosis factor alpha Cytotrophoblasts Signal transduction Receptor |
| Zdroj: | Biology of Reproduction. 55:400-409 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod55.2.400 |
| Popis: | Tumor necrosis factor alpha (TNF alpha) induces the apoptotic death of primary villous cytotrophoblasts in culture (Yui et al., Placenta 1994; 15:819). Since both p55 and p75 TNF receptors (TNFRs) localize to the villous trophoblast, we examined their roles in mediating trophoblast apoptosis. Comparison of 125I-TNF alpha binding competition by receptor-specific antibodies revealed 2.7-fold more TNFRp75 than TNFRp55. Immunohistochemical analysis of receptor distribution showed TNFRp75 to be expressed strongly in 40% but had little effect on TNFRp55 expression. Agonistic anti-TNFRp55 antibody and TNFRp55-specific TNF mutant protein stimulated both apoptosis and loss of trophoblast viability. In contrast, TNFRp75-specific mutant TNF alpha protein failed to induce either of these responses. Furthermore, neither cell death nor apoptosis stimulated by wild-type TNF alpha was inhibited by an antagonistic anti-TNFRp75 antibody. Thus, the apoptotic death of primary cytotrophoblasts is mediated almost entirely by TNFRp55, and the p75 receptor appears to have little effect on the process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|