SNHG1 Promotes Malignant Progression of Glioma by Targeting miR-140-5p and Regulating PI3K/AKT Pathway
| Autor: | Pengcheng Wang, Li-Li Xie, Hong-Tao Lv, Jian-Long Chen, Renduan Cai, Chuixue Huang, Chaocai Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Reporter gene Cell growth Chemistry Cell RNA Transfection medicine.disease 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology Apoptosis 030220 oncology & carcinogenesis Glioma Cancer research medicine PI3K/AKT/mTOR pathway |
| Zdroj: | Cancer Management and Research. 12:12011-12020 |
| ISSN: | 1179-1322 |
| DOI: | 10.2147/cmar.s269572 |
| Popis: | Purpose To explore the regulatory mechanism of long non-coding RNA small nucleolar RNA host gene 1 (SNHG1) in glioma. Materials and methods The expression of SNHG1 and miR-140-5p in glioma tissues and glioma cell lines (LN-18, KNS-81, and KALS-1) was determined, and the effect of the two on cell proliferation, invasion, and PI3K/AKT pathway was analyzed. Results SNHG1 was overexpressed in glioma tissues, while miR-140-5p was underexpressed in them, and there was a significant negative correlation between SNHG1 and miR-140-5p. In addition, both down-regulation of SNHG1 and up-regulation of miR-140-5p significantly inhibited the malignant proliferation and invasion of glioma, intensified the apoptosis, and also significantly suppressed the activation of the PI3K/AKT pathway. The dual-luciferase reporter assay, RNA pull-down assay, and RIP determination all confirmed that there was a targeting relationship between SNHG1 and miR-140-5p, and there was no difference between KNS-81 and KALS-1 cells transfected with SNHG1+mimics and si-SNHG1+inhibitor and those in the si-NC group with unrelated sequences in terms of cell malignant progression. Conclusion SNHG1/miR-140-5p axis and its regulation on PI3K/AKT pathway might be a novel therapeutic direction to curb the malignant progression of glioma. |
| Databáze: | OpenAIRE |
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