Bacterial DNA in the diagnosis of spontaneous bacterial peritonitis
| Autor: | Pere Coll, Germán Soriano, Eva Román, N. Calvo, Óscar Esparcia, Michel Montemayor, Carlos Guarner-Argente, Xavier Torras, R. Pericas, Ferran Navarro, Carlos Guarner |
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| Rok vydání: | 2010 |
| Předmět: |
Hepatology
biology business.industry Gastroenterology Peritonitis 16S ribosomal RNA medicine.disease biology.organism_classification law.invention Microbiology Spontaneous bacterial peritonitis law Ascites Immunology medicine Pharmacology (medical) medicine.symptom business Prospective cohort study Gene Polymerase chain reaction Bacteria |
| Zdroj: | Alimentary Pharmacology & Therapeutics. 33:275-284 |
| ISSN: | 0269-2813 |
| Popis: | Aliment Pharmacol Ther 2011; 33: 275–284 Summary Background Despite inoculation into blood culture bottles, ascitic fluid culture is negative in 50% of cases of spontaneous bacterial peritonitis (SBP). Aim To determine whether 16S rDNA gene detection by real-time polymerase chain reaction (PCR) and sequencing increases the efficacy of culture in microbiological diagnosis of spontaneous bacterial peritonitis. Methods We prospectively included 55 consecutive spontaneous bacterial peritonitis episodes in cirrhotic patients, 20 cirrhotic patients with sterile ascites and 27 patients with neoplasic ascites. Ascitic fluid was inoculated into blood culture bottles at the bedside and tested for bacterial DNA by real-time PCR and sequencing of 16S rDNA gene. Results Bacterial DNA was detected in 23/25 (92%) culture-positive SBP, 16/30 (53%) culture-negative SBP (P = 0.002 with respect to culture-positive SBP), 12/20 (60%) sterile ascites (P = 0.01 with respect to culture-positive SBP) and 0/27 neoplasic ascites (P |
| Databáze: | OpenAIRE |
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