Functional Characterization ofPsGPDin Drought Stress Response Using RNA-Seq Analysis of Transgenic Rice Plant
| Autor: | Kyong Mi Jun, Hyemin Lim, Gang-Seob Lee, Seung Uk Ji, Soo-Chul Park, Min Kang, So-Young Kim |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Abiotic component Abiotic stress Transgene fungi Drought tolerance food and beverages RNA-Seq Plant Science Biology 01 natural sciences Genetically modified rice 03 medical and health sciences Transformation (genetics) 030104 developmental biology Gene 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Breeding and Biotechnology. 8:131-140 |
| ISSN: | 2287-9366 2287-9358 |
| DOI: | 10.9787/pbb.2020.8.2.131 |
| Popis: | Plants are often exposed to biotic and abiotic stresses that affect plant growth, development, and productivity. Drought is an important abiotic stress that has a particularly serious impact on plant growth and development. We transformed rice with PsGPD using Agrobacterium-mediated transformation. We generated independent PsGPD-homozygous transgenic rice plants selected as single copy/intergenic lines by the TaqMan copy number assay and by T-DNA flanking sequences. These transgenic rice plants showed improvement of drought tolerance compared to wild-type plants under drought condition. RNA sequencing analysis showed that 2,992 genes were transcriptionally affected by the PsGPD transgene or drought treatment. In total, 145 genes were modulated by the PsGPD transgene before and after drought treatment. Among these candidate genes, 4 were up- and downregulated in all four comparisons. Several genes, including Os04t0576900, Os03t0629800, and Os04t0518400 (OsPAL7), were involved in tetrapyrrole synthesis. Os09t0522200 (DREB1A), an important component in hormone signal transduction, is a transcription factor (TF) gene that plays vital roles in stress responses. We partially characterized the functions of PsGPD in the drought stress response and the role of major TFs in the drought tolerance mechanism. These genes will be useful targets for both future research and the breeding of drought tolerance in rice. |
| Databáze: | OpenAIRE |
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