A sulfated polysaccharide κ-carrageenan induced antioxidant defense and proteomic changes in chloroplast against leaf spot disease of tomato
| Autor: | Sumithra Devi Mani, Mehanathan Muthamilarasan, Muthukumar Govindan, Radhakrishnan Nagarathnam |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification biology 010604 marine biology & hydrobiology fungi Acid phosphatase food and beverages Plant Science Aquatic Science Polysaccharide biology.organism_classification 01 natural sciences Septoria lycopersici Chloroplast chemistry.chemical_compound Kappaphycus alvarezii chemistry Biochemistry Chitin biology.protein Leaf spot 010606 plant biology & botany Peroxidase |
| Zdroj: | Journal of Applied Phycology. 33:2667-2681 |
| ISSN: | 1573-5176 0921-8971 |
| Popis: | Ecofriendly approaches to control plant diseases using biopolymers are gaining momentum due to their non-toxic nature to the environment. The present study elucidates the potency of a sulfated polysaccharide, κ-carrageenan, obtained from the red seaweed Kappaphycus alvarezii, as an inducer of antioxidant defense and also chloroplast proteome against leaf spot disease in tomato. к-carrageenan (0.3%) treatment was very effective in reducing disease scores in tomato plants. Fluorescent microscopy of tomato leaves stained with Uvitex 2B (a chitin specific stain) revealed that foliar application of κ-carrageenan (0.3%) reduced colonization of the pathogen Septoria lycopersici. In addition, κ-carrageenan pretreatment followed by pathogen infection recorded a higher accumulation of calcium oxalate crystals. The treatment of κ-carrageenan resulted in the induction of H2O2 and superoxide anion compared to control or S. lycopersici-infected plants. The tomato leaves treated with κ-carrageenan showed higher activity of peroxidase enzyme. Furthermore, chloroplast proteome analysis by 2D-PAGE of control, S. lycopersici-infected, κ-carrageenan-treated, and к-carrageenan treatment followed by S. lycopersici-infected leaves of tomato showed differential expression of 31 proteins, of which 11 proteins were upregulated including acid phosphatase 1-like, C- terminal processing peptidase, GATA transcription factor 5, Calvin cycle protein CP12-1, Photosystem I reaction center subunit PSAK, and subunit of cytochrome B6-F complex iron-sulfur centers. These results indicated that κ-carrageenan could be a promising inducer of antioxidant defense and modulator of chloroplast proteome in tomato plants. |
| Databáze: | OpenAIRE |
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