Transposon Mutagenesis of Foodborne Pathogenic Escherichia coli
| Autor: | Supraja Puttamreddy, F. Chris Minion |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Genetics
Transposable element 0303 health sciences 030306 microbiology Sequence analysis Biology biology.organism_classification medicine.disease_cause Enterobacteriaceae 03 medical and health sciences Pathogenic Escherichia coli medicine bacteria Transposon mutagenesis Escherichia coli 030304 developmental biology Sequence (medicine) |
| Zdroj: | Microbial Transposon Mutagenesis ISBN: 9781493995691 |
| DOI: | 10.1007/978-1-4939-9570-7_7 |
| Popis: | The Enterobacteriaceae, and in particular, Escherichia coli including foodborne pathotypes are particularly amenable to transposon mutagenesis. Here we describe the use of mini-Tn5 and Mu d1(Ap lac) to generate transposon inserts for analysis of enterohemorrhagic Escherichia coli EDL933. We also discuss how to array the library in 96-well plates and sequence individual clones for further analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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