Improvement of lipase productivity in bioprocesses using a structured mathematical model
| Autor: | Francisco Valero, M. A. Gordillo, José Luis Montesinos, Javier Lafuente, Carles Solà, Belkis Valdman |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
State variable
Chromatography biology Triacylglycerol lipase Substrate (chemistry) Bioengineering General Medicine Applied Microbiology and Biotechnology Dilution Candida rugosa Advanced Continuous Simulation Language biology.protein Lipase Bioprocess Biological system computer Biotechnology computer.programming_language |
| Zdroj: | Journal of Biotechnology. 52:207-218 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/s0168-1656(96)01646-x |
| Popis: | A simple structured mathematical model coupled with a methodology to estimate state variables and parameters was developed and applied to Candida rugosa batch and continuous lipase production. Process modelling was carried out using ACSL (Advanced Continuous Simulation Language). Once model parameters were determined, the whole model was validated, showing satisfactory results. In order to estimate the best strategy to improve lipase productivity, different simulations of batch, fed-batch and continuous cultures were made. The maximum enzyme productivity was predicted in continuous culture at moderate dilution rates (0.09 h −1 ) and substrate feeding concentration of 8 g l −1 . However, the highest predicted lipase activity was reached in fed-batch cultures with a prefixed substrate-feeding in order to maintain constant—at their optimal values—the relation substrate/biomass or the specific growth rate. The fed-batch process mode matched the simulation results. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect