Development of a novel microorganism immobilization method using anionic polyurethane

Autor: Wen-Chang Shih, Sz-Chwun John Hwang, Kuo-Cheng Chen, Yun-Huin Lin
Rok vydání: 2005
Předmět:
Zdroj: Journal of Applied Polymer Science. 99:738-743
ISSN: 1097-4628
0021-8995
Popis: Having the high abrasion resistance, hardness, extensibility, anti-lacerative power and low toxicity gave Anionic Polyurethane (PU) an advantage over the other synthetic materials. Many applications of immobilization used PU as the major media for microorganisms to grow. However, none of them was involved in using entrapped cell beads. This research contains the development of the PU-immobilized cell beads which must have a good machine characteristic, durability, inner and outside micro-structure, adsorption capability, and being functional. The best composition of PU-immobilized cell beads was made of PU of 51.43% to 53.33% in w/w as well as the alginate content of 0.11% to 0.57% in w/w. For machine characteristics, the strain gauge was employed to measure the maximum allowable gravity pressure. The PU-immobilized Strain F1 cell beads show about the same stress of 5.02 kgf/cm 2 as the control of 6.08 kgf/cm 2 . The whole experiment last at least 30 days, and no more than 0.4% of loss was measured. Compared to the PVA-immobilized cell beads, the better adsorption capability of the PU-immobilized cell beads is demonstrated for the hazardous chemical of toluene which was reduced by five sixth. Thus, the application of degrading hydrophobic compounds was expected. The functional tests were implemented by the application of biofiltration and fermentation. The high concentration of toluene (1500 ppm, v/v) was degraded within 5 hours. In addition, the high concentration of ethanol was produced from 100 g/L by using the PU-immobilized cell beads without any inhibition caused by the high concentration of ethanol. In order to have a porous structure in the PU-immobilized cell beads, adding vegetable oil to the PU/ALG solution can significantly reduce the PU content, which is helpful to improve the cost for immobilization. Adding air bubbles and starch into the PU solution also provide the cell beads with many micro-holes, which can be seen by SEM.
Databáze: OpenAIRE
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