Enzymic hydrolysis of rhizobial 4-O-methyl-d-glucurono-l-rhamnan
| Autor: | Linda K. Jackson, Martin C. Cadmus, Morey E. Slodki, William M. Rutherford, David Weisleder |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
chemistry.chemical_classification
Glycan Rhizobiaceae biology Enzymic hydrolysis Stereochemistry Chemistry Organic Chemistry General Medicine biology.organism_classification Biochemistry Analytical Chemistry Enzyme biology.protein Rhizobium Tetrasaccharide Polyacrylamide gel electrophoresis Bacteria |
| Zdroj: | Carbohydrate Research. 184:203-212 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/0008-6215(88)80018-1 |
| Popis: | A Bacillus sp. obtained from soil-enrichment culture excretes a depolymerase that degrades rhizobial 4-O-methylglucuronorhamnan. The enzyme was purified to homogeneity. According to SDS gel electrophoresis, it is a polypeptide chain of Mr ∼ 210,000. As determined by methylation analysis and n.m.r. spectroscopy, the sole product of enzymolysis is the linear tetrasaccharide 4-O-Me-β- d -GlcpA-(1→3)-α- l -Rhap-(1→3)-β- l -Rhap- (1→4)- l -Rha. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|