Biosynthesis of Tacrolimus by the Streptomyces tsukubensis VKM Ac-2618D Strain in the Presence of Polymeric Sorbents and Development of a Method for Its Isolation and Purification

Autor:	E. V. Titova, D. S. Salionov, V. Yu. Poshekhontseva, S. M. Staroverov, V. S. Karasev, A. A. Shutov, Marina V. Donova, V. M. Nikolaeva, G. G. Vasiarov, V. V. Fokina
Rok vydání:	2020
Předmět:	0106 biological sciences 0301 basic medicine Genus Streptomyces Sorbent Chromatography Strain (chemistry) Chemistry Silica gel chemical and pharmacologic phenomena 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Tacrolimus 03 medical and health sciences chemistry.chemical_compound surgical procedures operative 030104 developmental biology Biosynthesis 010608 biotechnology Yield (chemistry) Streptomyces tsukubensis
Zdroj:	Applied Biochemistry and Microbiology. 56:699-707
ISSN:	1608-3024 0003-6838
DOI:	10.1134/s0003683820060150
Popis:	Tacrolimus is known as the most effective immunosuppressive drug which is synthesized by actinobacteria of the genus Streptomyces. Its biosynthesis is accompanied by the formation of the structural analogs. A new method for the production of tacrolimus by Streptomyces tsukubensis BKM Ac-2618D with the use of brominated styrene-divinylbenzene sorbent SP-207 was developed. This approach enabled to increase the yield, prevent undesirable destruction, and simplify the tacrolimus isolation procedure from the cultivation media. The preliminary separation step was optimized by means of the usage of regenerable sorbents such as methylmethacrylate (HP2MG) and chemically modified silica gel (Diasorb-100-Diol). By implementation of the silica gel-based cation exchange resins pretreated with silver ions for the final purification stage, it was possible to increase its productivity. The overall yield of tacrolimus by this method was found to around 60–70%. The results could be implemented for the full-cycle production development of the pharmaceutical grade tacrolimus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4f65ca864c4e0c980966b5761a8ffa63 https://doi.org/10.1134/s0003683820060150 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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