Production of Single Contracting Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes: Matrigel Mattress Technique

Autor:	Matthew D. Durbin, Tromondae K. Feaster, Adrian G. Cadar, Charles C. Hong
Rok vydání:	2017
Předmět:	0301 basic medicine Matrigel Hipsc cms Cell Biology General Medicine 030204 cardiovascular system & hematology Biology Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Immunology Myocyte Stem cell Induced pluripotent stem cell Developmental Biology
Zdroj:	Current Protocols in Stem Cell Biology
ISSN:	1938-8969 1941-7322
Popis:	This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4f60f0a25afbed59183b575120f1c117 https://doi.org/10.1002/cpsc.32 Zobrazit plný text záznamu Plný text