Development and Validation of Difference Spectrophotometric Method for the Quantitative Estimation of Mesalamine in Bulk Drug and Dosage forms

Autor:	N. Raghavendra Babu, P. Ravi Kumar, P Niveditha, Y. Padmavathi
Rok vydání:	2017
Předmět:	Absorbance COX Inhibitor Chromatography Correlation coefficient Suppository Ich guideline Bulk drug Quantitative analysis (chemistry) Dosage form Mathematics
Zdroj:	Asian Journal of Pharmaceutical Analysis. 7:225
ISSN:	2231-5675 2231-5667
DOI:	10.5958/2231-5675.2017.00036.9
Popis:	A sensitive, precise, economic spectrophotometric method i.e. a difference spectroscopic method was developed for the estimation of mesalamine in bulk and dosage (tablet and suppository) forms. Difference spectroscopic method was developed by measuring the difference absorbance of the drug in 0.1N HCl and in 0.1 N NaOH showing maximum absorbance at 330nm. Beer's law was obeyed in the concentration range of 5–25 μg/ml. The LOD and LOQ were found to be 0.3 μg/ml and 0.9 μg/ml respectively. Correlation coefficient was observed as 0.999. The method was validated as per ICH guideline Q2R1 and the results were within the official limits. The developed difference spectroscopic method was applied for quantitative analysis of the drug in bulk and pharmaceutical dosage forms.
Databáze:	OpenAIRE
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