Candidate reference method for determination of total bilirubin in serum: development and validation

Autor:	P P Kwok-Cheung, B Jendrzejczak, Robert B. McComb, L L Hause, Billy W. Perry, Robert Schaffer, Basil T. Doumas
Rok vydání:	1985
Předmět:	Chromatography medicine.diagnostic_test Chemistry Bilirubin Biochemistry (medical) Clinical Biochemistry Standard solution Ascorbic acid Biological fluid chemistry.chemical_compound Biochemistry Reference values Spectrophotometry medicine Hemoglobin Albumin solution
Zdroj:	Clinical Chemistry. 31:1779-1789
ISSN:	1530-8561 0009-9147
Popis:	This candidate Reference Method for measuring total bilirubin in serum is based on the Jendrassik-Gróf principle (Clin Chem 29: 297-301, 1983). Standard Reference Material no. 916 bilirubin (National Bureau of Standards) is used as the standard. Bilirubin standard solutions may be prepared either in human serum or in 40 g/L albumin solution (human or bovine), because we found the molar absorptivity of the azopigment at 598 nm to be identical in these media. The absorptivities of the unconjugated and conjugated azopigments appear to be identical, but the conjugated azopigment is completely hydrolyzed in the final reaction mixture. Bilirubin added to serum from adults or neonates was quantitatively accounted for. Interference by hemoglobin (up to 2 g/L), ascorbic acid (up to 20 mg/L), or zinc (at physiological concentrations) is negligible. Of the therapeutic drugs we tested, only L-dopa and alpha-methyldopa interfere. We established normal adult reference values for total bilirubin and examined the intraindividual variation in 19 subjects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4f408ed9931a1f51786b3b2ecb0174b3 https://doi.org/10.1093/clinchem/31.11.1779 Zobrazit plný text záznamu