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A technique that yields conidial suspensions of Pyrenophora tritici-repentis nearly free of other propagules involves growing the fungus in potato-dextrose broth, followed by removal, comminution, and inoculation into liquid clarified V8 juice agar (CV8). After 48 hr of growth in the dark, the fungus is exposed to alternating periods of light and dark that stimulate the production of conidiophores and conidia in a lawn covering the entire surface of the colony. In two tests using 24 isolates of P. tritici-repentis, inoculum suspensions were obtained with an average of 1,386 and 1,692 propagules (conidia, conidiophores, and hyphal fragments) per milliliter, in which 92 and 100%, respectively, of the propagules were conidia |
