The effect of serum on the proliferation of bone marrow-derived mesenchymal stem cells from aged donors and donors with or without chronic heart failure
| Autor: | Noritoshi Nagaya, Hideki Kubo, Kanae Takamura, Hajime Ohgushi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty business.industry Mesenchymal stem cell Biomedical Engineering Medicine (miscellaneous) 030204 cardiovascular system & hematology Autologous serum medicine.disease Regenerative medicine Biomaterials Cell therapy 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Heart failure Internal medicine Immunology medicine Doubling time Bone marrow business Fetal bovine serum |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 12:e395-e397 |
| ISSN: | 1932-6254 |
| DOI: | 10.1002/term.2394 |
| Popis: | Many clinical studies of regenerative medicine using bone marrow-derived mesenchymal stem cells (MSCs) have been conducted globally. We initiated clinical studies using MSCs in 2001, and have now treated over 100 cases with patients aged 0–92 years. In a few cases involving patients with chronic heart failure (CHF), we observed that MSCs proliferated poorly. This contrasts with cell therapy studies wherein MSCs of patients with CHF were used for treatment. The effects of serum on the proliferation of MSCs from donors with normal heart function and with CHF have not been reported. Moreover, whether cell therapy is effective for elderly patients remains uncertain. Therefore, characterization of MSCs from aged donors and/or donors with CHF is urgently required. We retrospectively analyzed the population doubling times (PDTs) of MSCs between the first and second passages. Although we had data for many samples of well expanded MSCs from aged donors, a positive correlation was observed between donor age and PDT. A trend toward reduced variance in PDTs was observed in MSCs were supplemented with fetal bovine serum (FBS) compared to those supplemented with autologous serum. When autologous serum was used, the average PDT of MSCs from donors with CHF was significantly longer than that of MSCs from donors without CHF. In contrast, when FBS was used, similar PDTs were observed in MSCs from donors with and without CHF. Thus, FBS promotes MSC expansion even from donors with CHF, and that MSC-based regenerative medicine might be feasible even for elderly patients. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|