| Autor: |
Flaherty, Renée, Owen, Matthew, Fagan-Murphy, Aidan, Intabli, Haya, Healy, David, Patel, Anika, Allen, Marcus, Bhavik Patel, Flint, Melanie |
| Rok vydání: |
2017 |
| DOI: |
10.6084/m9.figshare.c.3726250_d2 |
| Popis: |
Stress hormones do not induce DNA damage or iNOS expression in a non-tumourigenic mammary epithelial cell line. a MCF10A cells were exposed to cortisol (1 μM) and NE (1 μM) for 30 minutes and assessed for DNA damage using the Comet assay. Comet tails indicating DNA strand breaks were visually scored according to intensity (0–4). Representative images shown. b MCF10A cells were exposed to cortisol (1 μM) for 30 minutes and 24 h and mRNA extracted. cDNA was synthesised and amplified in the presence of gene specific primers for NOS2 and β-actin using qRT-PCR. Ct values for NOS2 were normalised against β-actin and fold change calculated using the delta-Ct method. Mean ± SEM is expressed and significance was determined using one-way ANOVA (post hoc Tukey multiple comparisons); *significant increase, *p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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