The Physicochemical Characteristics of Serum Albumin and Erythrocyte Cell Membranes under Normal and Heart Failure Symptom Conditions
| Autor: | A. L. Dudylina, V. N. Titov, D. I. Grachev, Enno K. Ruuge |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology biology Biophysics Serum albumin Human serum albumin Microviscosity 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Membrane Blood serum chemistry Fatty acid binding biology.protein medicine lipids (amino acids peptides and proteins) Stearic acid Lipid bilayer medicine.drug |
| Zdroj: | Biophysics. 64:721-728 |
| ISSN: | 1555-6654 0006-3509 |
| DOI: | 10.1134/s0006350919050051 |
| Popis: | —Spin labeling EPR spectroscopy was used to study the structural and conformational characteristics of human serum albumin and human erythrocyte membranes under normal conditions and with symptoms of heart failure. 5-Doxyl stearic acid and 16-doxyl stearic acid were used as spin labels, whose paramagnetic NO fragments are bound to different sites of the hydrocarbon chain. The EPR spectra of 16-doxyl stearic acid indicate that in the physiological temperature range serum albumin molecules are characterized by several types of fatty acid binding sites, which differ in parameters of spin-label rotational diffusion. This distribution of fatty-acid binding sites was typical for the blood serum of all patients who participated in our study, regardless of deviations from the normal blood parameters. The microviscosity of erythrocyte membranes from patient blood was measured using both 5-doxyl stearic and 16-doxyl stearic spin labels, whose paramagnetic fragments are located at different depths inside the lipid bilayer. It was found that in patients with an increased erythrocyte distribution width, the membrane lipid microviscosity is statistically significantly higher than under normal conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink