A non-enzymatic nanomagnetic electro-immunosensor for determination of Aflatoxin B1 as a model antigen
| Autor: | Omid Sadeghi, Akram Shahrjerdi, Leila Masoomi, Mohammad Hossein Banitaba, Saied Saeed Hosseiny Davarani |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Detection limit
Aflatoxin Catechol Metals and Alloys Analytical chemistry Nanoparticle Condensed Matter Physics Electrochemistry Surfaces Coatings and Films Electronic Optical and Magnetic Materials Quinone Dielectric spectroscopy Chitosan chemistry.chemical_compound chemistry Materials Chemistry Electrical and Electronic Engineering Instrumentation Nuclear chemistry |
| Zdroj: | Sensors and Actuators B: Chemical. 177:1122-1127 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2012.11.067 |
| Popis: | A nonenzymatic sandwich-type electrochemical immunosensor was developed by means of a modified glassy carbon electrode: GCE/chitosan/Au nanoparticle (AuNP)/anti-Aflatoxin B 1 (a AFB 1 ), and a Fe 3 O 4 magnetic core with a gold shell functionalized with 3-((2-mercaptoethylimino)methyl)benzene-1,2-diol and labeled with an AFB 1 . The immunosensor was used for the detection of Aflatoxin B 1 as a model antigen. The current originated from the conversion of catechol to o -quinone and vice versa within a quasi-reversible two electrons–two protons reaction, considered as the response. The electrochemical studies demonstrated that the proposed immunoelectrode can be used to detect AFB 1 in the range of 0.6–110 ng mL −1 with a detection limit of 0.2 ng mL −1 . This immunosensor can be simply regenerated by using an external magnetic field which ensures a reproducible response. |
| Databáze: | OpenAIRE |
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