701 IN VITRO TESTS FOR CELL-MEDIATED IMMUNITY WITH PROTEIN EXTRACTS OF TISSUE ANTIGENS
| Autor: | Walter L Hanley, Zvi Aviner, Colette Severin |
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| Rok vydání: | 1978 |
| Předmět: | |
| Zdroj: | Pediatric Research. 12:480-480 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-197804001-00706 |
| Popis: | Good correlation of leucocyte migration inhibition (LMI) by human and animal corneal protein was reported in patients with keratopathies. Human and bovine tissue extracts were compared in 288 experiments for migration inhibitory factor (MIF) production and LMI in patients with various eye diseases. LMI and MIF were correlated with the in vivo skin test in patients suspected of immunodeficiencies and tuberculosis. Similar results were obtained in over 90% of experiments comparing MIF production to LMI using human and bovine ocular tissue antigens. Similar results were also obtained in over 90% of experiments comparing homologous to bovine ocular tissue antigens for MIF production and LMI. The in vitro tests were correlated with the in vivo skin test in patients tested for chronic mucocutaneous candidiasis and tuberculosis. The ocular tissue antigen concentration required in the LMI test is generally twice that required for MIF production. The in vivo skin test requires 48 hours for interpretation and remains the yardstick by which the in vitro tests are measured. It can be used with microbial antigens like candida and PPD but cannot be used with tissue protein antigens. |
| Databáze: | OpenAIRE |
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