Expression and Activity of the Na+/H+ Exchanger NHE-1 in Various Tissues of Spontaneously Hypertensive Rats and Normotensive Wistar-Kyoto Rats
| Autor: | Beate Quednau, Winfried Siffert, Norbert Haider, Dieter Rosskopf |
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| Rok vydání: | 1995 |
| Předmět: |
Gene isoform
medicine.medical_specialty Messenger RNA Vascular smooth muscle Physiology Chemistry musculoskeletal system Sodium–hydrogen antiporter Endocrinology Spontaneously hypertensive rat Internal medicine Gene expression cardiovascular system medicine cardiovascular diseases Northern blot Steady state (chemistry) circulatory and respiratory physiology |
| Zdroj: | Cellular Physiology and Biochemistry. 5:276-285 |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Vascular smooth muscle cells (VSMC) from the spontaneously hypertensive rat (SHR) exhibit an enhanced Na+/H+ exchange activity which coincides with increased proliferation. The aim of the present work was to investigate Na+/H+ exchange activity in VSMC and thymocytes from SHR and Wistar-Kyoto (WKY) rats and to examine the steady state levels of specific Na+/H+ exchanger (isoform 1; NHE-1) mRNA transcripts in VSMC, thymocytes, heart, and kidney.VSMC from SHR proliferated significantly faster than those from WKY rats. NaVH+ exchange activity (Vmax) was significantly (p + × liter–1 × min–1; mean ± SE). In contrast, Na+/H+ exchange activity was higher in thymocytes from WKY rats. By Northern blot analysis, we observed similar steady state mRNA levels for NHE-1 in tissues from SHR and WKY, respectively.We conclude that the enhanced Na+/H+ exchange activity found in VSMC from SHR is not expressed in all tissues of SHR. Furthermore, our data suggest that over-expression of NHE-1 is not responsible for the enhanced Na+/ H+ exchange activity in VSMC from SHR. Thus, alterations in cell signalling located upstream of the Na+/H+ exchanger may induce both an increased proliferation and an altered post-translational regulation of Na+/H+ exchange activity in VSMC from SHR. |
| Databáze: | OpenAIRE |
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