Heterogeneous firing rate response of mouse layer V pyramidal neurons in the fluctuation-driven regime
| Autor: | Bartosz Telenczuk, Alain Destexhe, Gilles Ouanounou, Yann Zerlaut, Charlotte Deleuze, Thierry Bal |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Membrane potential education.field_of_study Quantitative Biology::Neurons and Cognition Sensory processing Physiology Sodium channel medicine.medical_treatment Population Time constant Biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Visual cortex medicine.anatomical_structure medicine Asynchronous network Soma education Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Physiology. 594:3791-3808 |
| ISSN: | 0022-3751 |
| Popis: | Key points We recreated in vitro the fluctuation-driven regime observed at the soma during asynchronous network activity in vivo and we studied the firing rate response as a function of the properties of the membrane potential fluctuations. We provide a simple analytical template that captures the firing response of both pyramidal neurons and various theoretical models. We found a strong heterogeneity in the firing rate response of layer V pyramidal neurons: in particular, individual neurons differ not only in their mean excitability level, but also in their sensitivity to fluctuations. Theoretical modelling suggest that this observed heterogeneity might arise from various expression levels of the following biophysical properties: sodium inactivation, density of sodium channels and spike frequency adaptation. Abstract Characterizing the input–output properties of neocortical neurons is of crucial importance for understanding the properties emerging at the network level. In the regime of low-rate irregular firing (such as in the awake state), determining those properties for neocortical cells remains, however, both experimentally and theoretically challenging. Here, we studied this problem using a combination of theoretical modelling and in vitro experiments. We first identified, theoretically, three somatic variables that describe the dynamical state at the soma in this fluctuation-driven regime: the mean, standard deviation and time constant of the membrane potential fluctuations. Next, we characterized the firing rate response of individual layer V pyramidal cells in this three-dimensional space by means of perforated-patch recordings and dynamic clamp in the visual cortex of juvenile mice in vitro. We found that individual neurons strongly differ not only in terms of their excitability, but also, and unexpectedly, in their sensitivities to fluctuations. Finally, using theoretical modelling, we attempted to reproduce these results. The model predicts that heterogeneous levels of biophysical properties such as sodium inactivation, sharpness of sodium activation and spike frequency adaptation account for the observed diversity of firing rate responses. Because the firing rate response will determine population rate dynamics during asynchronous neocortical activity, our results show that cortical populations are functionally strongly inhomogeneous in young mouse visual cortex, which should have important consequences on the strategies of cortical computation at early stages of sensory processing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text